Gene synthesis is the foundation of the new field of Synthetic Biology, and is powering novel findings across research disciplines. These disciplines enhance our knowledge of biological processes and generate products which greatly improve our lives, fast-tracking our abilities in vaccine production, gene therapy, data storage and CRISPR editing.
Gene synthesis can construct a wide variety of types of DNA sequences without a template, simplifying the creation of DNA tools that can be produced to aid diverse research applications.
However, many high-value target sequences (inserts) that come from gene synthesis are greater than 3 kbp (30,000 bp) in length. This level of complexity greatly complicates synthesis and increases the risk of introducing impurities into the process.
Introducing Ranger® Size Selection
10X Improvement in efficiency
Due to the inherent potential for error in each step of gene synthesis, all synthetic sequences should be verified before use.
When longer DNA constructs are needed, Ranger® Technology can help greatly improve the efficiency of this critical step, drastically reducing the turnaround time and revolutionising the traditional approach to gene synthesis.
Size Selection is about enriching and purifying; preferentially selecting material of interest by differentiating based on size. We can use size selection to minimise noise associated with truncation products, recovering the full length DNA construct of interest only.
Ranger® Size Selection employs machine vision in scalable electrophoresis to:
- Affirm insert purity
- Isolate inserts from off-target impurities
Graphic shows :Using machine vision and voltage control, Ranger® employs dynamic voltage adjustment across all channels for synchronised arrival of desired fragment sizes at extraction wells.
Join the Ranger® Revolution
Yourgene Health offer a range of platforms that are powered by Ranger® Technology.
Regardless of your gene synthesis needs, we have a solution for you:
A Ranger® Technology Application: Synthetic Biology and Gene Synthesis
Read about how dozens of organisations are already benefitting from using Ranger® Technology’s size selection function for synthetic biology and gene synthesis applications in our latest Your Expert article.
Co-Creator of Ranger®, Matthew Nesbitt, discusses how Ranger® Technology can help overcome issues in traditional gene synthesis and synthetic biology applications.
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