Yourgene® Insight DPYD – Just launched!
The right assay makes the difference
Go beyond standard DPYD testing with Insight – We are proud to launch the newest version of our DPYD genotyping kit that incorporates the latest global guideline recommendations of expanded variant coverage.
One tube, more variants.
Read the press release here.
Introducing Yourgene® Insight DPYD
The Yourgene® Insight DPYD kit uses fluorescent ARMS (Amplification Refractory Mutation System) allele specific amplification technology, which detects point mutations, insertions or deletions in DNA, to deliver accurate, rapid detection of 19 key DPYD variants with a fast turnaround time of only 4 hours! For a full list variants see the table below.
Categorisation of DPYD variants
| Tier One | Tier Two | Other DPYD Variants |
|---|---|---|
|
c.1905+1G>A
|
c.299_302del
|
c.496A>G
|
|
c.1679T>G
|
c.703C>T
|
c.2043_2058del
|
|
c.1129-5923C>G,
c.1236G>A |
c.1314T>G
|
c.2194G>A
|
|
c.557a>G
|
c.1475C>T
|
c.601A>C
|
|
c.868A>G
|
c.1774C>T
|
c.2872A>G
|
|
c.2279C>T
|
c.2639G>T
|
|
|
c.2846A>T
|
|
|
Key Benefits of Yourgene® Insight DPYD
- Comprehensive variant detection providing enhanced global coverage
- Highly multiplexed two-tube assay with ready to use reagents – just add DNA
- Fast results with a 4-hour turnaround time
- Delivers high-quality reliable results
Yourgene® Insight DPYD Workflow
- Simple, streamlined workflow
- High throughput flexibility
- Cost and time-efficient
- Easy data analysis
- Secure, local data interpretation
- No specialised equipment required
Yourgene® Insight DPYD kit technical details
| Parameter | Format |
|---|---|
|
Details
|
Two-tube assay:
- Tube 1 detects the 19 defined variants - Tube 2 detects the corresponding wildtype sequence to detect heterozygotes - STR markers are included in each tube to aid sample identification |
|
Parameter
|
PCR method
|
|
Details
|
ARMS-PCR
|
|
Parameter
|
Hands on time
|
|
Details
|
<60 mins
|
|
Parameter
|
Total turnaround time
|
|
Details
|
PCR: ~3 hoursCapillary Electrophoresis: ~1 hour
|
|
Parameter
|
Intended sample type
|
|
Details
|
DNA extracted from whole human blood (EDTA preserved)
|
|
Parameter
|
Instrumentation
|
|
Details
|
Thermal Cycler & Capillary Electrophoresis Genetic Analysers. Developed on ABI 3500 and SeqStudio CE instruments
|
|
Parameter
|
Software analysis
|
|
Details
|
GeneMapper™ or GeneMarker®
|
Seraseq® DPYD DNA Mutation Mix
You can now buy reference material from us when purchasing Yourgene® Insight DPYD kits.
We stock the Seraseq® DPYD DNA Mutation Mix (RUO), which is compatible with the Yourgene® Insight DPYD Assay (RUO).
Please note, the Seraseq® DPYD DNA Mutation Mix is sold separately to the Yourgene® Insight DPYD assay. If you wish to order the reference materials, please ask your local sales rep or distributor.
Please note, it is the customer’s responsibility to fully validate the Seraseq® DPYD DNA Mutation Mix before use.
Implementing DPYD Genotyping into Clinical
Practice
In this edition of Your Expert, Yourgene Health interviewed Dr. Daniel Hertz, Associate Professor at the University of Michigan College of Pharmacy, about the importance of DPYD testing, it’s role as a standard of care, and the ongoing challenges to broader clinical adoption.
